Accu Test HIV 1/2 Ab Test Kit

Overview Accu Test HIV 1/2 Ab Test Kit

The AccuTest HIV 1/2 Ab Test Kit is a rapid, visual immunoassay for the qualitative in-vitro detection of HIV-1 and HIV-2 antibodies in human serum or plasma. It aids in HIV infection diagnosis, but a positive result necessitates confirmatory testing within a validated HIV testing protocol. HIV, a retrovirus targeting immune cells, leads to Acquired Immunodeficiency Syndrome (AIDS) in its advanced stages. Standard HIV detection involves enzyme immunoassay (EIA) followed by confirmatory methods. This AccuTest kit offers a simple, visual, 15-minute qualitative result based on immunochromatography. Its application is the detection of HIV-1 and HIV-2 antibodies in serum/plasma. The device utilizes a lateral flow cassette format employing colloidal gold-conjugated recombinant HIV-1 (gp120 + gp41) and HIV-2 (GP-36) antigens immobilized on a nitrocellulose strip. Goat Anti-Mouse IgG antibodies are present at the control zone. Sample migration rehydrates the conjugate; the presence of HIV antibodies binds to the conjugate, migrating to the test zone (1 or 2) where capture by corresponding antigens forms visible purple lines. Absence of antibodies results in a lack of lines in the test zone; the control line, formed by conjugate capture at the control zone, validates the test. Samples with high red blood cell or fibrin concentrations should be recentrifuged. Intended for in-vitro diagnostics only, this test indicates antibody presence, not definitive HIV diagnosis; further testing (e.g., rapid tests, EIA, Western blotting) is crucial for confirmation. Results should be interpreted alongside clinical data and do not define HIV serotype. Do not mix components from different lots. Allow all components to reach room temperature (15-30°C) before use. After removing the device from its foil pouch, immediately add one drop (10µl) of sample and two drops (approx. 75µl) of diluent to the sample well; interpret results at 15-20 minutes, but not after 20 minutes. Store unopened at 4-30°C; do not freeze. Protect from humidity. Do not use beyond the expiration date or if the pouch is damaged. Handle specimens as potentially infectious. The kit is sensitive to heat and humidity. Use immediately after opening. Read all instructions carefully. Keep out of reach of children. For external use only.

How to Use Accu Test HIV 1/2 Ab Test Kit:

Before testing, equilibrate the assay device, buffer, and sample to ambient temperature (15-30°C). Immediately upon removing the device from its protective foil, and within one hour, place it on a stable, dry surface and clearly label it with the specimen identifier. Using a vertical dispensing technique, carefully introduce a single drop (10µl) of the specimen into the designated sample well, followed by two drops (approximately 75µl) of the provided reagent. Initiate the timer. Observe the lateral migration of purple coloration across the central detection zone as the assay proceeds. Allow sufficient time for color development. Read and record the results between 15 and 20 minutes post-addition of reagent. Results obtained after 20 minutes are invalid.

Safety Instruction of Accu Test HIV 1/2 Ab Test Kit:

• Keep the test kit in its unopened pouch at a temperature between 4 and 30 degrees Celsius; refrigeration is acceptable. Avoid freezing. Shield the kit from moisture.
• The device remains stable until the expiration date shown on the kit and/or its sealed packaging.
• Discard after the expiry date.
• Keep the testing device in its unopened package until needed.
• Optimal outcomes are achieved by following the given directions.
• Treat all samples as if they could transmit infection.
• The instrument's performance is affected by both temperature and moisture levels.
• Discard the test kit if its packaging is compromised or the seal is breached.
• Apply the diagnostic device directly upon its removal from the packaging.
• This kit's components—the test device and assay diluents—have undergone standard batch quality control testing.
• Always check the product label before administering.
• Store in a location inaccessible to children.
• Apply topically only.