URIT 10G Urine Reagent Strips

Overview URIT 10G Urine Reagent Strips

URIT 10G urine reagent strips offer semi-quantitative analysis of urinary leukocytes, ketones, nitrites, urobilinogen, bilirubin, glucose, protein, specific gravity, pH, blood, ascorbic acid, microalbumin, calcium, and creatinine. Each strip features reagent-impregnated pads and a reagent-free calibration area on a plastic backing, enabling multi-analyte testing for routine diagnostics and population screenings. The calibration pad automatically compensates for urine's natural color, ensuring accurate instrumental readings. Applications include: semi-quantitative assessment of leukocytes, ketones, nitrites, urobilinogen, bilirubin, protein, glucose, specific gravity, blood, and pH in urine samples. **Product Specifications and Performance Characteristics:** * **Leukocytes:** Detects granulocyte esterases via an indoxyl esterase reaction, producing a violet dye. Lysed leukocytes may yield positive results despite absent cells. Vaginal discharge can cause false positives in female samples. High glucose (≥55mmol/L) or specific gravity may reduce readings. Cephalexin, cephalothin, and tetracycline can decrease reactivity, potentially leading to false negatives. Lymphocytes are not detected. Temperature influences reactivity. * **Ketones:** Employs a Legal's test variant, showing greater sensitivity to acetoacetic acid than acetone. β-hydroxybutyric acid does not react. High specific gravity/low pH urine may yield Trace results. Highly pigmented urine or urine containing significant levodopa metabolites may cause false positive (Trace) readings. * **Nitrites:** Based on the Griess reaction, detecting nitrite specifically. Any pink coloration indicates a positive result, suggesting ≥10⁵ organisms/mL, though color intensity doesn't correlate with bacterial count. A negative result doesn't exclude significant bacteriuria. False negatives can arise from organisms lacking nitrate reductase, insufficient bladder retention (<8 hours), or dietary nitrate deficiency. Ascorbic acid ≥1.4mmol/L can mask nitrite (≤43µmol/L). * **Urobilinogen:** Uses the Ehrlich reaction, detecting ≥3µmol/L (≈0.2 Ehrlich unit/dL). Interfering substances reacting with Ehrlich's reagent may lead to false positives. Red-colored pigments or medications can also interfere. Formaldehyde inhibits the reaction. Optimal temperature is 22-26°C. Absence of urobilinogen cannot be definitively determined. * **Bilirubin:** Relies on diazonium salt coupling in acid medium. Even trace amounts are clinically significant. Certain urine constituents (medications, indicators) may produce yellow/red discoloration, hindering interpretation. Ascorbic acid ≥1.4mmol/L may cause false negatives. * **Glucose:** Utilizes a glucose oxidase/peroxidase reaction, specific to glucose. Ascorbic acid >1.4mmol/L and/or high ketone levels (8mmol/L) can produce false negatives with low glucose (5.5mmol/L). High urine specific gravity reduces reactivity. Hypochlorite or peroxide contamination can cause false positives. Temperature affects reactivity. * **Protein:** Employs a protein error of indicators principle, showing greater sensitivity to albumin. High pH (up to 9) can affect results. Quaternary ammonium or chlorhexidine disinfectant residues may cause false positives. * **Specific Gravity:** A detergent and bromthymol blue indicator detects ionic constituents, with color change from green to yellow (1.005-1.030 range). * **pH:** A mixed indicator provides a clear color change between pH 5.0 and 9.0. * **Blood:** Hemoglobin/myoglobin catalyze indicator oxidation using hydroperoxide. Highly sensitive to hemoglobin, complementing microscopy. High specific gravity may reduce sensitivity. Equally sensitive to myoglobin and hemoglobin (150-520 mg/L ≈ 5-15 RBCs/µL). Captopril and iodine can decrease reactivity. Menstruation and oxidizing contaminants (hypochlorite) can cause false positives. Microbial peroxidase may yield false positives. Ascorbic acid ≥1.4mmol/L can cause false negatives at trace levels. * **Microalbumin:** More sensitive to albumin than globulins, hemoglobin, Bence-Jones protein, or mucin; a negative result doesn't exclude these. 20-200mg/L indicates microalbuminuria; >200mg/L indicates clinical albuminuria. Little interference from creatinine or hemoglobin. High urine concentration and alkaline urine may cause false positives. * **Calcium:** Based on calcium ion reaction with OCPC, producing a color change. High magnesium levels may interfere. * **Creatinine:** Uses a reaction with 3,5-dinitrobenzoic acid, producing a color change. Daily excretion is relatively constant. Certain compounds and high yellow pigment concentrations may interfere. Microalbumin-to-creatinine ratio reduces stochastic error; normal ratio <3.4mg/mmol, abnormal 3.4-33.9mg/mmol, highly abnormal >33.9mg/mmol. **Instructions for Use:** Requires URIT-50,180, 5008, 500C, 330, 31, 560 urine analyzer. Fully immerse the strip in well-mixed urine (sample tube ≥88mm), ensuring all pads are wetted (2 seconds). Remove excess urine by dragging the strip along the container rim and blotting. Avoid cross-contamination between pads. Follow analyzer instructions for interpretation. **Safety Precautions:** For healthcare professionals only. Visual interpretation is not recommended; color chart is for reference only. Use fresh urine in a clean, dry container. Protect from sunlight to prevent bilirubin and urobilinogen oxidation. Avoid touching reagent pads. Keep desiccant in place; replace cap promptly after use. Do not use expired or deteriorated strips. Return strips to room temperature before testing. Oxidizing disinfectant residues can cause false positives for blood and glucose. Do not add preservatives; avoid volatile chemical contamination. Dispose of used strips appropriately.

How to Use URIT 10G Urine Reagent Strips:

Necessary supplies: URIT-50,180, 5008, 500C, 330, 31, 560 urine dipstick. Submerge the reagent pads fully in a homogenized urine sample; the sample's level must exceed 88mm. Ensure complete pad wetting. After a 2-second immersion, withdraw the strip. The drip technique is also acceptable. Gently wipe the strip's edge against the container rim to eliminate excess urine. Then, blot the strip lengthwise on absorbent paper, preventing cross-contamination between reagent areas. Consult the device's instructions for interpreting results with the urine analyzer.

Safety Instruction of URIT 10G Urine Reagent Strips:

• This assay is for use by qualified medical personnel only.
• This strip is not intended for visual assessment; the colors on the strip are for identification purposes only, and should not be used in evaluating the test results.
• Obtain a new urine sample using a clean, dry collection vessel. Avoid exposing the urine sample to direct sunlight, as this causes bilirubin and urobilinogen oxidation, resulting in falsely reduced measurements for these analytes.
• Avoid hand contact; maintain reagent strip reaction areas in a clean, uncontaminated state.
• Keep the desiccant packets in place. Securely replace the container lid after each reagent strip removal; prolonged exposure to humid air (over five minutes) may compromise test accuracy.
• Discard reagent strips past their expiration date; avoid using damaged, faded, or darkened strips.
• Bring to room temperature prior to administration.
• Inaccurate blood and glucose test results may arise from the presence of highly reactive disinfectant remnants in the sample container. Urine samples should be preservative-free. Prevent contamination from volatile substances.
• Discard used test strips; they are single-use and must be treated as medical waste.